{"id":4049,"date":"2025-03-14T06:42:23","date_gmt":"2025-03-14T06:42:23","guid":{"rendered":"http:\/\/legatobio.com\/?p=4049"},"modified":"2025-03-14T06:42:23","modified_gmt":"2025-03-14T06:42:23","slug":"diturbo-empowered-stable-cell-line-development","status":"publish","type":"post","link":"\/?p=4049","title":{"rendered":"DiTurbo\u2122 Empowered Stable Cell Line Development"},"content":{"rendered":"<figure class=\"wp-block-post-featured-image\"><img width=\"2356\" height=\"1702\" src=\"\/app\/uploads\/2025\/03\/image6.webp\" class=\"attachment-post-thumbnail size-post-thumbnail wp-post-image\" alt=\"\" decoding=\"async\" loading=\"lazy\" srcset=\"\/app\/uploads\/2025\/03\/image6.webp 2356w, \/app\/uploads\/2025\/03\/image6-300x217.webp 300w, \/app\/uploads\/2025\/03\/image6-1024x740.webp 1024w, \/app\/uploads\/2025\/03\/image6-768x555.webp 768w, \/app\/uploads\/2025\/03\/image6-1536x1110.webp 1536w, \/app\/uploads\/2025\/03\/image6-2048x1479.webp 2048w\" sizes=\"(max-width: 2356px) 100vw, 2356px\" \/><\/figure>\n\n\n<p>Legato Biologics offers comprehensive Cell Line Development (CLD) services, leveraging robust host cell lines such as CHO K1 and CHO K1 GS-KO to support diverse biopharmaceutical applications. Utilizing the proprietary DiTurbo\u2122 dual-vector transfection system<b>, <\/b>Legato Biologics executes efficient gene expression and accelerated cell line establishment, ensuring high productivity and stability. The company&#8217;s streamlined development workflow, achieving PCB (Production Cell Bank) generation in 3 to 4 months from DNA sequence, reflects its commitment to speed and efficiency in bioprocess development.<\/p>\n<p>With a proven track record of 20+ successful cell line development projects, Legato Biologics consistently achieves high product titers, up to <b>10 g\/L<\/b>, making it a preferred partner for both early-stage and late-stage biologics development. The company&#8217;s high-throughput platform facilitates the selection of optimal cell lines tailored for fed-batch processes with robust performance and scalability. Our data-driven approach enables precise cell line selection to meet specific production needs and regulatory requirements.<span class=\"Apple-converted-space\">\u00a0<\/span><\/p>\n<p>Legato Biologics\u2019 CLD capabilities emphasize<b> <\/b>rapid and reliable recombinant cell line development<b> <\/b>with a strong focus on clonality assurance. The integration of a <b>single-cell imager<\/b> and <b>high-throughput detector<\/b> allows thorough characterization and documentation of clonality, fulfilling regulatory expectations and accelerating the transition to clinical and commercial manufacturing. These advanced tools empower Legato Biologics to deliver high-quality, scalable solutions that support the evolving demands of the biopharmaceutical industry.<\/p>\n<p>Legato Biologics&#8217;s CLD capabilities:<\/p>\n<ul>\n<li>Robust host cell lines, including CHO K1 and CHO K1 GS-KO, optimized for high-yield and stable protein expression.<\/li>\n<li>DiTurbo\u2122 dual-vector transfection system, enabling efficient gene delivery and rapid cell line establishment with enhanced productivity.<\/li>\n<li>Accelerated development timeline, achieving production cell bank (PCB) generation in 3 to 4 months from DNA sequence.<\/li>\n<li>Proven expertise with over 20 successful cell line development projects, achieving product titers of up to 10 g\/L.<\/li>\n<li>High-throughput screening platform for precise selection of cell lines optimized for fed-batch production processes.<\/li>\n<li>Rapid and reliable recombinant cell line development with a clonality guarantee, supported by state-of-the-art single-cell imaging and high-throughput detection technologies.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Legato Biologics offers comprehensive Cell Line Development (CLD) services, leveraging robust host cell lines such as CHO K1 and CHO K1 GS-KO to support diverse biopharmaceutical applications. Utilizing the proprietary DiTurbo\u2122 dual-vector transfection system, Legato Biologics executes efficient gene expression and accelerated cell line establishment, ensuring high productivity and stability.<\/p>\n","protected":false},"author":1,"featured_media":4023,"comment_status":"closed","ping_status":"","sticky":false,"template":"","format":"standard","meta":[],"categories":[54],"tags":[],"_links":{"self":[{"href":"\/index.php?rest_route=\/wp\/v2\/posts\/4049"}],"collection":[{"href":"\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=4049"}],"version-history":[{"count":1,"href":"\/index.php?rest_route=\/wp\/v2\/posts\/4049\/revisions"}],"predecessor-version":[{"id":4050,"href":"\/index.php?rest_route=\/wp\/v2\/posts\/4049\/revisions\/4050"}],"wp:featuredmedia":[{"embeddable":true,"href":"\/index.php?rest_route=\/wp\/v2\/media\/4023"}],"wp:attachment":[{"href":"\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=4049"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=4049"},{"taxonomy":"post_tag","embeddable":true,"href":"\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=4049"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}